Journal: Nature Communications

Article Title: CD1d-dependent rewiring of lipid metabolism in macrophages regulates innate immune responses

doi: 10.1038/s41467-022-34532-x

Figure Lengend Snippet: a qPCR expression data of Cd36 and Msr1 in WT and CD1d-KO cells ( n = 4–6). b Western-blots and quantification for CD36 and β-actin in WT and CD1d-KO BMDCs ( n = 3). c Flow cytometry images and quantification of Mean fluorescence intensity (MFI) for CD36 and MSR1 in WT and CD1d-KO cells ( n = 3–13). * p < 0.05, *** p < 0.001, one-sample t -test. d Confocal microscopy images showing staining of CD1d (red), CD36 (green) and DAPI (blue) for WT ( n = 123) and CD1d-KO BMDCs ( n = 91). Graphs show fluorescence intensity for CD1d and CD36 (left) and Pearson correlation coefficient (right). Scale bar is 20 μm. **** p < 0.0001 two-tailed unpaired t -test. e PLA was performed in WT and CD1d-KO BMDCs for CD1d and CD36. Representative images of PLA (yellow) and DAPI (blue) and quantification of PLA (puncta per cell) are shown ( n = 162–305 cells). Scale bar is 7 μm. **** p < 0.0001 two-tailed unpaired t -test. f Flow-cytometry analyses of surface CD36 in WT and CD1d-KO pMacs after incubation with oxLDL (left). MFI for CD36 (middle) or values relative to control (untreated) cells (right) are shown ( n = 6). ** p < 0.01; *** p < 0.001; **** p < 0.0001, 2-way ANOVA. g Flow-cytometry analyses of CD36 in WT pMacs pre-treated with αCD1d or isotype (iso) and incubated with oxLDL ( n = 9). CD36 levels are shown relative to control (untreated cells + isotype). * p < 0.05; paired two-tailed t -test. h Uptake of DiI-acLDL in pMacs pre-treated with αCD1d or isotype ( n = 4). ** p < 0.01, unpaired two-tailed t -test. i BMDCs were cultured with αCD1d or isotype. qPCR data show relative expression of the depicted genes ( n = 9). ** p < 0.01 one-sample t -test. j BMDCs were cultured with αCD1d or isotype (iso) and stimulated with LPS ( n = 5–9). Expression of the depicted genes was measured by qPCR. * p < 0.05, ** p < 0.01 one-sample t -test. k Uptake of DiI-acLDL in HEK293T cells transfected with CD1d, mutant CD1d (CD1d-TD or CD1d-Y) and/or CD36 ( n = 4). Some samples were incubated with αCD1d as indicated. * p < 0.05; ** p < 0.01; *** p < 0.001, one-way ANOVA. Bars in all graphs represent mean +/− SEM. Values for n represent biologically independent samples (as shown by the number of data points in each graph). n = cells isolated from individual mice ( a–c , f – j ), cells ( d , e ) or transfections ( k ). Source data are provided as a Source Data file.

Article Snippet: The plasmid containing mouse CD36 (pCMV3-HA) was purchased from SinoBiological.

Techniques: Expressing, Western Blot, Flow Cytometry, Fluorescence, Confocal Microscopy, Staining, Two Tailed Test, Incubation, Cell Culture, Transfection, Mutagenesis, Isolation

Journal: iScience

Article Title: Angiogenesis-independent VEGF signaling enhances exercise capacity by increasing fat oxidation in mice fed sulfur amino acid-restricted diets

doi: 10.1016/j.isci.2025.114148

Figure Lengend Snippet: Transcriptomics across muscle depots reveal metabolic shift from glycolytic toward oxidative (A) Experimental set up and color scheme used throughout and . (B) Fold changes of transcripts associated with fatty acid (FA) catabolism and transport as identified in supplementary Extended Data A in muscle of male mice ( n = 6) given ad libitum access to sulfur amino acid-restricted (SAAR) versus control (Con) diet for seven days. (C) Pathway enrichment analysis of genes showing significant diet-by-muscle interaction effects. (D) Fold changes (SAAR vs. Con) of TCA cycle genes in EDL and soleus. (E) Representative blots of electron transport chain complexes. (F) Quantification of relative protein abundance normalized to vinculin of SDHB for EDL and soleus ( n = 6) of male mice given ad libitum access to SAAR versus Con diet on day seven. (G) Representative blots of CD36, LPL, and PDK4 and vinculin ( top ) and quantification of relative protein abundance normalized to vinculin of CD36, PDK4, and LPL ( bottom ) in EDL ( n = 6) of male mice given ad libitum access to SAAR versus Con diet on day seven. (H) Representative blots of CD36, LPL, and PDK4 and vinculin ( top ) and quantification of relative protein abundance normalized to vinculin of CD36, PDK4, and LPL from blots ( bottom ) in soleus ( n = 6) of male mice given ad libitum access to SAAR versus Con diet on day seven. All panels represent data from mice that were not subjected to endurance running. All data are shown as mean, and error bars indicate SD unless otherwise noted; p values indicate the significance of the difference by Student t test between diets; significance is determined by p < 0.05. See also and . Raw data files for E are available in the supplement as ZIP file.

Article Snippet: Mouse: CD36 fl/fl , Charles River Laboratories , N/A.

Techniques: Control, Quantitative Proteomics

Journal: iScience

Article Title: Angiogenesis-independent VEGF signaling enhances exercise capacity by increasing fat oxidation in mice fed sulfur amino acid-restricted diets

doi: 10.1016/j.isci.2025.114148

Figure Lengend Snippet: SAAR increases muscle lipid flux without altering lipid pool sizes (A) Experimental design and color scheme used in A–3E and A. (B) Circulatory carbon flux ( n = 10–13) of 13 C 18 -U-Linolate of jugular-vein-catheterized male mice given ad libitum access to sulfur amino acid-restricted (SAAR) versus control (Con) diet for seven days. (C) Ex vivo β-oxidation measured by incorporation of 3 H-palmitic acid in 3 H-H 2 O in muscles of male mice fed a Con or SAAR diet for seven days ( n = 15). (D) Representative fluorescence images ( left ) of BODIPY 493:503 (green), WGA647 (red), and DAPI (blue) staining in EDL cross sections (scale bar, 50 μm) and quantification of Bodipy + Intensity within fibers ( right ) of male mice fed a Con or SAAR for seven days ( n = 6). (E) Lipidomics analysis from muscle of male mice fed a Con or SAAR diet for seven days ( n = 6), summarized as normalized ion counts of each main lipid class. (F) Experimental set up and color scheme used in G, 3H, and D–S3I. (G) Percent change in body weight ( n = 16) of male WT and EC CD36−/− mice given ad libitum access to SAAR versus Con diet after seven days. (H) Distance ran during a one-time maximal endurance test (n = 9–15) of male WT and EC CD36−/− mice given ad libitum access to SAAR versus Con diet on day seven. B–E represent data from mice that were not subjected to endurance running, G and H represent data from mice subjected to maximal endurance testing. All data are shown as mean, and error bars indicate SD unless otherwise noted; p values indicate the significance of the difference by Student t test between diets, or two-way ANOVA with Sidak multiple comparisons test between diets and muscle or genotype; significance is determined by p < 0.05. See also and , , and .

Article Snippet: Mouse: CD36 fl/fl , Charles River Laboratories , N/A.

Techniques: Control, Ex Vivo, Muscles, Fluorescence, Staining

Journal: Journal of Cachexia, Sarcopenia and Muscle

Article Title: A negative feedback loop between fibroadipogenic progenitors and muscle fibres involving endothelin promotes human muscle fibrosis

doi: 10.1002/jcsm.12974

Figure Lengend Snippet: Antibody panel used for CyTOF

Article Snippet: CD36 , 152Sm , 5‐271 , Fluidigm , 3152007B.

Techniques: